Neutrophil elastase-cleaved corticosteroid-binding globulin is absent in human plasma
نویسندگان
چکیده
منابع مشابه
Pseudomonas aeruginosa elastase disrupts the cortisol-binding activity of corticosteroid-binding globulin.
The serine protease inhibitor (SERPIN) family member corticosteroid-binding globulin (CBG) is the main carrier of glucocorticoids in plasma. Human CBG mediates the targeted release of cortisol at sites of inflammation through cleavage of its reactive center loop (RCL) by neutrophil elastase. The RCLs of SERPIN family members are targeted by diverse endogenous and exogenous proteases, including ...
متن کاملResidues in the human corticosteroid-binding globulin reactive center loop that influence steroid binding before and after elastase cleavage.
Corticosteroid-binding globulin (CBG) is a non-inhibitory serine proteinase inhibitor (serpin) that transports cortisol and progesterone in blood. Crystal structures of rat CBG and a thrombin-cleaved human CBG:anti-trypsin (Pittsburgh) chimera show how structural transitions after proteolytic cleavage of the CBG reactive center loop (RCL) could disrupt steroid binding. This ligand release mecha...
متن کاملSpecific binding of human corticosteroid-binding globulin to cell membranes.
Specific binding sites for corticosteroid-binding globulin were detected on membranes prepared from human prostates. The binding sites are typical of membrane receptors: they are saturable and specific and have high affinity. There was little specific binding at 4 degrees C and 23 degrees C. Maximal specific binding was obtained at 37 degrees C. Scatchard analysis revealed the presence of a sin...
متن کاملExpression of corticosteroid-binding globulin CBG in the human heart.
Glucocorticoids are known to be involved in myocardial regeneration and destruction. Cardiomyocytes are mostly devoid of nuclear glucocorticoid receptors (GRs) and it is generally assumed that effects of adrenal steroids in heart are mediated through the mineralocorticoid receptor (MR). Here we used immunocytochemistry to study localization of corticosteroid binding globulin (CBG) in semithin s...
متن کاملGlycosylation of human corticosteroid-binding globulin at aspargine 238 is necessary for steroid binding.
Human corticosteroid binding-globulin (CBG) is a plasma glycoprotein that binds and regulates the biological activity of glucocorticoids and progesterone. Carbohydrates comprise approximately 25% of its molecular mass being represented by bi- and triantennary N-linked oligosaccharides of the N-acetyllactosamine type. To assess the impact of these carbohydrate chains on CBG production and steroi...
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ژورنال
عنوان ژورنال: Journal of Endocrinology
سال: 2019
ISSN: 0022-0795,1479-6805
DOI: 10.1530/joe-18-0479